Rapid and Sensitive Liquid Chromatography-tandem Mass Spectrometric Method for the Simultaneous Estimation of the Antiasthematic Drug Salbutamol Sulphate and Beclomethasone Dipropionate, in Human Plasma

A highly sensitive, specific and rapid LC–ESI-MS/MS method has been developed and validated for simultaneous quantification of Salbutamol and Beclomethasone in human plasma (200 μl) using Anastrozole respectively, as an internal standard (IS) as per the regulatory guidelines. Results: The LLE method was used to extract the analytes and IS from human plasma. The chromatographic resolution of Salbutamol, Beclomethasone and Anastrozole IS was achieved using an isocratic flow on a RP8 column. The total chromatographic run time was 3.5 min. A linear response function was established for the range of concentrations 23 to 4000 ng/mL and 8 to 1500 ng/mL for Salbutamol and Beclomethasone, respectively, in human plasma. Conclusion: The intraand inter-day accuracy and precision values for Salbutamol and Beclomethasone met the acceptance as per regulatory guidelines.